Difference between revisions of "Part:BBa K2916009"

Latest revision as of 02:47, 22 October 2019

EF-Tu protein equipped with a 6x HIS affinity tag

Elongation Factor Tu is a protein that is used in the PURE and OnePot PURE cell-free systems.

Usage and Biology

Elongation factors are a set of proteins that function during the second step of protein biosynthesis, the elongation phase. They act upon the ribosome, to facilitate translational elongation from formation of the first to the last peptide bond of a growing polypeptide. The elongation phase is the most rapid step in translation, so the factors also regulate the procedure happening during that step to ensure high accuracy of the translation.

The Elongation Factor Tu in particular mediates the entry of the aminoacyl-tRNA into a free site of the ribosome

In our part, besides the sequence encoding for the protein we also have a hexahistidine-tag to allow us purify the protein.

In our project we used EF-Tu as a part of the protein solution needed for OnePot PURE cell-free system using the method of gravity flow affinity chromatography, as described in the protocol we designed. In fact EF-Tu accounts for 47% of the final protein concentration on the OnePot PURE protein solution.

Characterization

All the data regarding the characterization of that part can be found in the composite part: BBa_K2916038 .

Sequence and Features