Difference between revisions of "Part:BBa K102912"

 
 
Line 1: Line 1:
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K102912 short</partinfo>
 
<partinfo>BBa_K102912 short</partinfo>
Line 11: Line 10:
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K102912 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K102912 SequenceAndFeatures</partinfo>
 +
 +
'''Data sheet'''
 +
 +
[[Image:NINT_Mfold-K102912.jpg|center|800px]]
  
  

Latest revision as of 01:46, 27 October 2008

TA12 plus pause sequence

This design incorporate stem bulges and YUNR sequence in the loop (5' UUGG) as per Isaacs et al, 2004 to improve RNA stability and susceptibility to anti-sense induction. In addition a "pause" sequence (5' atgtgtgct) is added at the 3' end of the stem.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 14
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Data sheet

NINT Mfold-K102912.jpg