Difference between revisions of "Part:BBa K2992013"
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===Usage=== | ===Usage=== | ||
− | T<i>fad</i> is a terminator sequence derived from the <i>fad</i> gene of <i>C. tetani</i>, encoding FAD-binding oxidreducatse. These enzymes catalyse the conversion of dihydroxyacetone phosphate to glycerol-3-phosphate in a reversible reaction. In our project we use this strong terminator to prevent polar transcription from the <i>pyrKDE</i> operon of <i>C. sporogenes</i>, following chromosomal knock-in of <i>botR</i> in part [https://parts.igem.org/Part:BBa_K2992025], at this locus. The secondary structure for T<i>fad</i> was predicted using the Mfold web tool (Zuker, 2003). | + | T<i>fad</i> is a terminator sequence derived from the <i>fad</i> gene of <i>C. tetani</i>, encoding FAD-binding oxidreducatse. These enzymes catalyse the conversion of dihydroxyacetone phosphate to glycerol-3-phosphate in a reversible reaction. In our project we use this strong terminator to prevent polar transcription from the <i>pyrKDE</i> operon of <i>C. sporogenes</i>, following chromosomal knock-in of <i>botR</i> in part [https://parts.igem.org/Part:BBa_K2992025 BBa_K2992025], at this locus. The secondary structure for T<i>fad</i> was predicted using the Mfold web tool (Zuker, 2003). |
__NOTOC__ | __NOTOC__ | ||
{|width='80%' | {|width='80%' | ||
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===Characterisation=== | ===Characterisation=== | ||
− | This basic part was used | + | This basic part was used upstream of P<i>botR</i> to prevent chromosomal read-through from the <i>pyrKDE</i> operon, upon integration of the P<i>botR</i>-<i>botR</i> module at this locus. See our [https://2019.igem.org/Team:Nottingham/Results results page] for more information. |
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<br> | <br> | ||
− | + | <span class='h3bb'>Sequence and Features</span> | |
+ | <partinfo>BBa_K2992013 SequenceAndFeatures</partinfo> | ||
===References=== | ===References=== |
Latest revision as of 02:52, 22 October 2019
Usage
Tfad is a terminator sequence derived from the fad gene of C. tetani, encoding FAD-binding oxidreducatse. These enzymes catalyse the conversion of dihydroxyacetone phosphate to glycerol-3-phosphate in a reversible reaction. In our project we use this strong terminator to prevent polar transcription from the pyrKDE operon of C. sporogenes, following chromosomal knock-in of botR in part BBa_K2992025, at this locus. The secondary structure for Tfad was predicted using the Mfold web tool (Zuker, 2003).
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Tfad from C. tetani
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Secondary Structure
Characterisation
This basic part was used upstream of PbotR to prevent chromosomal read-through from the pyrKDE operon, upon integration of the PbotR-botR module at this locus. See our results page for more information.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
Zuker, M. (2003). Mfold web server for nucleic acid folding and hybridization prediction. Nucleic Acids Research, 31(13), pp.3406-3415.
Measurement
- [http://openwetware.org/wiki/Cconboy:Terminator_Characterization/Results How these parts were measured]