Difference between revisions of "Part:BBa K157027"

 
 
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__NOTOC__
 
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<partinfo>BBa_K157027 short</partinfo>
 
<partinfo>BBa_K157027 short</partinfo>
  
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Part consists of the signal peptide of Erbb1/EGF-receptor, which ensures the transport to the cell membrane scFV-antiNIP,  GGGSflexlinker (extracellular parts), the trans-membrane region of the Erbb1/EGF-receptor and the N-terminal part of the split-fluorophor YFP(intracellular part).
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Part consists of the signal peptide of Erbb1/EGF-receptor, scFV-antiNIP,  GGGSflexlinker, the trans-membrane region of the Erbb1/EGF-receptor and the N-terminal part of the split-fluorophor YFP.<br>
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Part was derived by cloning of BBa_K157001, BBa_K157003, BBa_K157013, BBa_K157002, BBa_K157008.<br>
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For further information see the description of the parts mentioned above.<br>
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 17:19, 27 October 2008

SP-AntiNIP-GGGSlinker-Tm-nYFP

Part consists of the signal peptide of Erbb1/EGF-receptor, scFV-antiNIP, GGGSflexlinker, the trans-membrane region of the Erbb1/EGF-receptor and the N-terminal part of the split-fluorophor YFP.
Part was derived by cloning of BBa_K157001, BBa_K157003, BBa_K157013, BBa_K157002, BBa_K157008.
For further information see the description of the parts mentioned above.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 868
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4
    Illegal AgeI site found at 1423
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 12