Difference between revisions of "Part:BBa K3087000:Design"
 
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===Design Notes===
 
===Design Notes===
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Needed to be complementary to other oligo from parts BBa_K3087001, BBa_K3087002 and BBa_K3087003 to form the Lactobacillus targeting nano-structure.
+
It is needed to be complementary to other oligo from parts BBa_K3087001, BBa_K3087002 and BBa_K3087003 to form the Lactobacillus targeting nano-structure.
  
 
The design of the Lactobacillus targeting nano-structure plasmid are as follow:
 
The design of the Lactobacillus targeting nano-structure plasmid are as follow:
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[[File:T--HK SKHLPSS-plasmidss.png|500px]]
 
[[File:T--HK SKHLPSS-plasmidss.png|500px]]
  
−
The biobricks contained in our plasmid are illustrated in the above graph. Four basic parts we designed, including BBa_K3087000, BBa_K3087001,BBa_K3087002and BBa_K3087003, are the DNAs synthesized to form our Lactobacillus targeting nano-structure (LTN).
+
The biobricks contained in our plasmid are illustrated in the above graph. Four basic parts we designed, including BBa_K3087000, BBa_K3087001,BBa_K3087002 and BBa_K3087003, are the DNAs synthesised to form our Lactobacillus targeting nano-structure (LTN).
  
 
The promoter used in these four basic parts is J23100 and the terminator used is B0054. HIV Terminal Binding Site (HTBS) used in each plasmid encode the end of our desired gene. The binding of HIV reverse transcriptase (HIVRT) would then reversely transcribe our target DNA.
 
The promoter used in these four basic parts is J23100 and the terminator used is B0054. HIV Terminal Binding Site (HTBS) used in each plasmid encode the end of our desired gene. The binding of HIV reverse transcriptase (HIVRT) would then reversely transcribe our target DNA.
  
  
 +
[[File:T--HK_SKHLPSS--validation.jpeg ‎|300px]]
  
−
===Source===
+
The validation of plasmid is shown above. O1, O2, O3 and O4 represent the part BBa_K3087000, BBa_K3087001, BBa_K3087002 and BBa_K3087003 respectively.
  
−
This is synthesized artificially based on the DNA sequence of Lactobacillus. It is not from any genome sequence.
+
The information of the four basic parts are as follow:
 +
[[File:T--HK_SKHLPSS--parts_information.jpeg ‎|700px]]
 +
 
 +
===Source===
  
 +
This is a newly synthesised sequence which is artificially designed based on the DNA sequence of Lactobacillus.
 
===References===
 
===References===
  
 
https://parts.igem.org/wiki/index.php?title=Part:BBa_K3087000:Design&action=submit
 
https://parts.igem.org/wiki/index.php?title=Part:BBa_K3087000:Design&action=submit

Latest revision as of 03:02, 22 October 2019

_NOTOC_ Strand 1 for formation of Lactobacillus targeting nano-structure (LTN)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 13
    Illegal NheI site found at 36
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It is needed to be complementary to other oligo from parts BBa_K3087001, BBa_K3087002 and BBa_K3087003 to form the Lactobacillus targeting nano-structure.

The design of the Lactobacillus targeting nano-structure plasmid are as follow:

T--HK SKHLPSS-plasmidss.png

The biobricks contained in our plasmid are illustrated in the above graph. Four basic parts we designed, including BBa_K3087000, BBa_K3087001,BBa_K3087002 and BBa_K3087003, are the DNAs synthesised to form our Lactobacillus targeting nano-structure (LTN).

The promoter used in these four basic parts is J23100 and the terminator used is B0054. HIV Terminal Binding Site (HTBS) used in each plasmid encode the end of our desired gene. The binding of HIV reverse transcriptase (HIVRT) would then reversely transcribe our target DNA.


T--HK SKHLPSS--validation.jpeg

The validation of plasmid is shown above. O1, O2, O3 and O4 represent the part BBa_K3087000, BBa_K3087001, BBa_K3087002 and BBa_K3087003 respectively.

The information of the four basic parts are as follow: T--HK SKHLPSS--parts information.jpeg

Source

This is a newly synthesised sequence which is artificially designed based on the DNA sequence of Lactobacillus.

References

https://parts.igem.org/wiki/index.php?title=Part:BBa_K3087000:Design&action=submit