Difference between revisions of "Part:BBa K3208010"

 
 
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This part encodes a red expression cassette that can be inserted between two Eco31I sites. This modification can significantly decrease the number of colonies that have to be screened to find a correct transformant when using vectors that rely on Eco31I digestion and ligation.
 
This part encodes a red expression cassette that can be inserted between two Eco31I sites. This modification can significantly decrease the number of colonies that have to be screened to find a correct transformant when using vectors that rely on Eco31I digestion and ligation.
  
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NB!!! This part is NOT designed for a BioBrick assembly!!! Its purpose is to serve as a PCR matrix. In order to insert it between the two Eco31I sites, one needs to amplify it with primers with corresponding overhangs (due to the specifics of class IIs restriction enzymes like Eco31I). Use Snapgene to plan your correct cloning strategy
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NB!!! This part is NOT designed for a BioBrick assembly!!! Its purpose is to serve as a PCR matrix. In order to insert it between the two Eco31I sites, one needs to amplify it with primers with corresponding overhangs (due to the specifics of class IIs restriction enzymes like Eco31I). Use Snapgene to plan your correct cloning strategy.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 23:37, 21 October 2019


Red expression cassette

This part encodes a red expression cassette that can be inserted between two Eco31I sites. This modification can significantly decrease the number of colonies that have to be screened to find a correct transformant when using vectors that rely on Eco31I digestion and ligation.


NB!!! This part is NOT designed for a BioBrick assembly!!! Its purpose is to serve as a PCR matrix. In order to insert it between the two Eco31I sites, one needs to amplify it with primers with corresponding overhangs (due to the specifics of class IIs restriction enzymes like Eco31I). Use Snapgene to plan your correct cloning strategy.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 787
    Illegal AgeI site found at 899
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1036
    Illegal BsaI.rc site found at 1