Difference between revisions of "Part:BBa K3117012"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | The secretion of the recombinant protein into the periplasma has distinct advantages like the oxidising environment for the formation of disulfide bonds, which does | + | The secretion of the recombinant protein into the periplasma has distinct advantages like the oxidising environment for the formation of disulfide bonds, which does hardly occur in the reducing environment of the cytoplasm. Additionally, foldases inside the periplasm catalyse the formation and isomerisation of disulfide bonds (Schlegel et al. 2013). |
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Latest revision as of 01:25, 22 October 2019
pelB signal sequence
pelB is a signal sequence, which can target recombinant proteins to the periplasmic space. It uses the post-translational translocation pathway (Singh et al. 2013).
Usage and Biology
The secretion of the recombinant protein into the periplasma has distinct advantages like the oxidising environment for the formation of disulfide bonds, which does hardly occur in the reducing environment of the cytoplasm. Additionally, foldases inside the periplasm catalyse the formation and isomerisation of disulfide bonds (Schlegel et al. 2013).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 54
- 1000COMPATIBLE WITH RFC[1000]