Difference between revisions of "Part:BBa K2916073:Design"

(→‎Source)
 
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===Source===
 
===Source===
  
−
The toehold has incorporated as an overhand on the primer designed to amplify sfGFP and the composite part was produced with PCR.
+
Order toehold as primers and perform PCR on the repressed gene plasmid.  
  
 
===References===
 
===References===
  
 
Green, Alexander A., et al. “Toehold Switches: De-Novo-Designed Regulators of Gene Expression.” Cell, vol. 159, no. 4, Nov. 2014, pp. 925–39. DOI.org (Crossref), doi:10.1016/j.cell.2014.10.002.
 
Green, Alexander A., et al. “Toehold Switches: De-Novo-Designed Regulators of Gene Expression.” Cell, vol. 159, no. 4, Nov. 2014, pp. 925–39. DOI.org (Crossref), doi:10.1016/j.cell.2014.10.002.

Latest revision as of 02:14, 22 October 2019


Toehold switch for Bois Noir 2.1 with superfolder GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 96
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 141


Source

Order toehold as primers and perform PCR on the repressed gene plasmid.

References

Green, Alexander A., et al. “Toehold Switches: De-Novo-Designed Regulators of Gene Expression.” Cell, vol. 159, no. 4, Nov. 2014, pp. 925–39. DOI.org (Crossref), doi:10.1016/j.cell.2014.10.002.