Difference between revisions of "Part:BBa K3192030:Design"
 
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===Design Notes===
 
===Design Notes===
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This part was designed using DeNovo DNA software to optimize translation initial rates
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<p>Denovo DNA was used for design of the Ribosomal Binding site. Given the coding sequence for the specified gene, DeNovo ran thousands of iterations to develop a RBS with high translation initiation rates. This sequence was then synthesized (for the 2019 Virginia iGEM team it was synthesized with its respective coding region). Sequencing confirmed the identity of the synthesized construct. </p>
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===References===
 
===References===
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https://www.denovodna.com:4433

Latest revision as of 01:36, 22 October 2019


Custom RBS Optimzed for BBa_K3192018


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 4
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Denovo DNA was used for design of the Ribosomal Binding site. Given the coding sequence for the specified gene, DeNovo ran thousands of iterations to develop a RBS with high translation initiation rates. This sequence was then synthesized (for the 2019 Virginia iGEM team it was synthesized with its respective coding region). Sequencing confirmed the identity of the synthesized construct.


Source

Synthetic

References

https://www.denovodna.com:4433