Difference between revisions of "Part:BBa K136015:Design"

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===Design Notes===
 
===Design Notes===
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pBad/araC was digested by SpeI -PstI  (insert) and cloned into the psb2k3 that contain the gfp tripart .
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gfp tripart (backward insert) was cloned into pSB2K3 containing pBad/araC.
  
 
===Source===
 
===Source===

Latest revision as of 14:50, 26 October 2008

pBad/araC - GFP tripart


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1883
    Illegal SapI site found at 961


Design Notes

gfp tripart (backward insert) was cloned into pSB2K3 containing pBad/araC.

Source

flgA promoter comes from E. coli K12 strain MG1655. gfp tripart comes from the registry.

References