Difference between revisions of "Part:BBa K117004:Experience"
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The NTU@iGEM team characterized the biobrick part pLacI-GFP. The influence of 1)concentration of Lactose 2) temperature and 3) time were investigated. | The NTU@iGEM team characterized the biobrick part pLacI-GFP. The influence of 1)concentration of Lactose 2) temperature and 3) time were investigated. | ||
| + | [[image:NTU@iGEM_Diagram2_of_characterization_pLacI-GFP.JPG|thumb|center|400px| The characterization overlook]] | ||
Shown below is the graph obtained for the experiment conducted at 37°C. | Shown below is the graph obtained for the experiment conducted at 37°C. | ||
[[image:NTU@iGEM_37norm.jpg|thumb|center|400px| Characterization at 37°C]] | [[image:NTU@iGEM_37norm.jpg|thumb|center|400px| Characterization at 37°C]] | ||
Latest revision as of 01:01, 27 October 2008
Contents
Abnormality found in the registry
This device could be found in the registry in the form of BBa_J04430. However, the team found an abnormality when BBa_J04430 was transformed into Top 10 cells. As shown on the pictures below, the cells glow pink instead of green under UV light.
.JPG)
Characterization of pLacI-GFP
The NTU@iGEM team characterized the biobrick part pLacI-GFP. The influence of 1)concentration of Lactose 2) temperature and 3) time were investigated.
Shown below is the graph obtained for the experiment conducted at 37°C.
Electron microscope pictures
For more information including the protocols and results obtained, please go to:
[http://2008.igem.org/Team:NTU-Singapore/Parts/Characterization_of_LacI-GFP NTU@iGEM Characterization of pLac-GFP]
User Reviews
UNIQa41fd4597f8d9f68-partinfo-00000000-QINU UNIQa41fd4597f8d9f68-partinfo-00000001-QINU