Difference between revisions of "Part:BBa K3046005:Design"
 
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===Design Notes===
 
===Design Notes===
 
This part is optimised to be compatible with the MoClo standard, Mobius assembly standard and not to contain any SwaI cut sites.
 
This part is optimised to be compatible with the MoClo standard, Mobius assembly standard and not to contain any SwaI cut sites.
−
 
  
 
===Source===
 
===Source===
 
This part is created on the basis of the upstream region of the gpdA ORF (Systematic ORF name: An12g07450) of many <i>aspergillus</i> genomes. It has been created using the proHMMoter software with added noise, as a part of the LEAP project, described in further detail here: https://2019.igem.org/Team:DTU-Denmark/Model
 
This part is created on the basis of the upstream region of the gpdA ORF (Systematic ORF name: An12g07450) of many <i>aspergillus</i> genomes. It has been created using the proHMMoter software with added noise, as a part of the LEAP project, described in further detail here: https://2019.igem.org/Team:DTU-Denmark/Model
−
 
  
 
===References===
 
===References===
 
[1] P. Schape et al., “Updating genome annotation for the microbial cell factory Aspergillus niger using gene co-expression networks.,” Nucleic Acids Res., vol. 47, no. 2, pp. 559–569, Jan. 2019.
 
[1] P. Schape et al., “Updating genome annotation for the microbial cell factory Aspergillus niger using gene co-expression networks.,” Nucleic Acids Res., vol. 47, no. 2, pp. 559–569, Jan. 2019.

Latest revision as of 20:35, 21 October 2019


PLEAPmstA_1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 61
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 470
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is optimised to be compatible with the MoClo standard, Mobius assembly standard and not to contain any SwaI cut sites.

Source

This part is created on the basis of the upstream region of the gpdA ORF (Systematic ORF name: An12g07450) of many aspergillus genomes. It has been created using the proHMMoter software with added noise, as a part of the LEAP project, described in further detail here: https://2019.igem.org/Team:DTU-Denmark/Model

References

[1] P. Schape et al., “Updating genome annotation for the microbial cell factory Aspergillus niger using gene co-expression networks.,” Nucleic Acids Res., vol. 47, no. 2, pp. 559–569, Jan. 2019.