Difference between revisions of "Part:BBa K3174008:Design"

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==Conception and Design==
 
==Conception and Design==
  
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The Austin_UTexas 2015 iGEM team originally identified that the SYFP2 gene was especially prone to IS10 element mutations (See their work <html><a href="http://2015.igem.org/Team:Austin_UTexas/Project/Plasmid_Study">here</a></html>). Kelsey Hu then performed the initial cloning and characterization of the redesigned sequence. Later the Austin_UTexas 2019 iGEM team retrieved the sequence and Anna Bardenhagen performed propagation experiments replicating Austin_UTexas 2015's <html><a href="http://2015.igem.org/Team:Austin_UTexas/Project/Strain_Study">procedure</a></html> for both the original and redesigned SYFP2 sequences.
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The Austin_UTexas 2015 iGEM team originally identified that the SYFP2 gene was especially prone to IS10 element mutations (See their work <html><a href="http://2015.igem.org/Team:Austin_UTexas/Project/Plasmid_Study">here</a></html>). Kelsey Hu then performed the initial cloning and characterization of the redesigned sequence.  
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Later the Austin_UTexas 2019 iGEM team retrieved the sequence and Anna Bardenhagen performed propagation experiments replicating Austin_UTexas 2015's <html><a href="http://2015.igem.org/Team:Austin_UTexas/Project/Strain_Study">procedure</a></html> for both the original and redesigned SYFP2 sequences. This data was then used to fully characterize the stability of the new part as it compared to the original. A registry page was created to document the sequence and characterization of the new part.
  
 
===Design Notes===
 
===Design Notes===
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The redesigned sequence must be less prone to IS10 insertion element mutations while still mainting the same amino acid sequence.
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The redesigned sequence must be less prone to IS10 insertion element mutations while still mainting the same amino acid sequence. This was achieved by altering the sequence at positions 30-41 from <html>aggcgtagtaccg to <b>t</b>gg<b>g</b>gt<b>t</b>gt<b>t</b>cc<b>a</b>.</html>
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===Source===
 
===Source===

Latest revision as of 21:26, 21 October 2019


SYFP2 coding sequence with IS hotspot removed


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Conception and Design

The Austin_UTexas 2015 iGEM team originally identified that the SYFP2 gene was especially prone to IS10 element mutations (See their work here). Kelsey Hu then performed the initial cloning and characterization of the redesigned sequence.
Later the Austin_UTexas 2019 iGEM team retrieved the sequence and Anna Bardenhagen performed propagation experiments replicating Austin_UTexas 2015's procedure for both the original and redesigned SYFP2 sequences. This data was then used to fully characterize the stability of the new part as it compared to the original. A registry page was created to document the sequence and characterization of the new part.

Design Notes

The redesigned sequence must be less prone to IS10 insertion element mutations while still mainting the same amino acid sequence. This was achieved by altering the sequence at positions 30-41 from aggcgtagtaccg to tggggttgttcca.

Source

The original SYFP2 coding sequence (BBa_K864100)

References