Difference between revisions of "Part:BBa K3285700"

 
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pbrD is a protein that sequesters lead. It is present downstream of a strong RBS (BBa_B0034) and binds to lead ions in the cytoplasm and decrease its concentration.
 
pbrD is a protein that sequesters lead. It is present downstream of a strong RBS (BBa_B0034) and binds to lead ions in the cytoplasm and decrease its concentration.
  
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===Usage and Biology===
 
===Usage and Biology===
  
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===Construction===
 
===Construction===
  
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"https://2019.igem.org/wiki/images/d/d2/T--IISER-Pune-India--pbrD_Gel.jpeg"
! [[file:pbrD_Gel.jpeg|200px|]]
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The part was artificially synthesised with corresponding primers that have overlap with GFP sequence. This allows it to be put downstream of the lead dependent biosensor [https://parts.igem.org/Part:BBa_K1758333 BBa_K1758333]. This part has been synthesised with a strong Ribosome Binding Site [https://parts.igem.org/Part:BBa_B0034 BBa_B0034] upstream of the coding sequence.  
The part was artificially synthesised with corresponding primers that have overlap with GFP sequence. This allows it to be put downstream of the lead dependent biosensor (<a href="https://parts.igem.org/Part:BBa_K1758333">BBa_K1758333</a>). This part has been synthesised with a strong Ribosome Binding Site (<a href="https://parts.igem.org/Part:BBa_B0034">BBa_B0034</a>) upstream of the coding sequence.  
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Latest revision as of 17:49, 21 October 2019


Lead Sequestration Protein with RBS

pbrD is a protein that sequesters lead. It is present downstream of a strong RBS (BBa_B0034) and binds to lead ions in the cytoplasm and decrease its concentration.

Usage and Biology

This is an intermediate part which can be incorporated under a promoter. The pbrD protein is known to sequester lead ions in the cytoplasm of the bacterial cell.

When put under a lead responsive promoter, the part is expected to show dosage dependent sequestration properties.

Construction

"T--IISER-Pune-India--pbrD_Gel.jpeg"

The part was artificially synthesised with corresponding primers that have overlap with GFP sequence. This allows it to be put downstream of the lead dependent biosensor BBa_K1758333. This part has been synthesised with a strong Ribosome Binding Site BBa_B0034 upstream of the coding sequence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 532
  • 1000
    COMPATIBLE WITH RFC[1000]