Difference between revisions of "Part:BBa K2956000"
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This is a composite part consisting of a strong promoter, ribosome binding site, and the gene for flavone synthase (FNS). When inserted into a vector and transformed in E. coli, this part allows for the constitutive expression of FNS. FNS converts naringenin to apigenin via a direct 2,3-desaturation of flavanones. | This is a composite part consisting of a strong promoter, ribosome binding site, and the gene for flavone synthase (FNS). When inserted into a vector and transformed in E. coli, this part allows for the constitutive expression of FNS. FNS converts naringenin to apigenin via a direct 2,3-desaturation of flavanones. | ||
| − | + | [[File:T--NYU_New_York--FNS1.png|780px|thumb|left|Figure 1: Conversion of Naringenin to Apigenin in FNS B, measured by gas chromatography]] | |
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Latest revision as of 17:41, 21 October 2019
RBS-FNS under J23100 Promoter
This is a composite part consisting of a strong promoter, ribosome binding site, and the gene for flavone synthase (FNS). When inserted into a vector and transformed in E. coli, this part allows for the constitutive expression of FNS. FNS converts naringenin to apigenin via a direct 2,3-desaturation of flavanones.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 794
- 1000COMPATIBLE WITH RFC[1000]