Difference between revisions of "Part:BBa K3110011"
| (One intermediate revision by one other user not shown) | |||
| Line 5: | Line 5: | ||
This construct has L-Lactate Dehydrogenase (lldD) under the control of a weak promoter and a medium RBS. | This construct has L-Lactate Dehydrogenase (lldD) under the control of a weak promoter and a medium RBS. | ||
lldD cleaves L-lactate to pyruvate. Our intention of using this part is to have control over the detection threshold of L-Lactate by the lldPRD regulatory region. We have also designed other parts which produce various higher and lower concentrations of lldD by varying the strength of the promoter and RBS controlling its production. | lldD cleaves L-lactate to pyruvate. Our intention of using this part is to have control over the detection threshold of L-Lactate by the lldPRD regulatory region. We have also designed other parts which produce various higher and lower concentrations of lldD by varying the strength of the promoter and RBS controlling its production. | ||
| − | |||
| − | |||
| − | |||
| − | |||
| Line 23: | Line 19: | ||
<h1>Characterization </h1> | <h1>Characterization </h1> | ||
| − | Due to shortage of time we could not characterize this part | + | Due to shortage of time we could not characterize this part. |
Latest revision as of 20:59, 21 October 2019
Weak Promoter Medium RBS lldD
This construct has L-Lactate Dehydrogenase (lldD) under the control of a weak promoter and a medium RBS. lldD cleaves L-lactate to pyruvate. Our intention of using this part is to have control over the detection threshold of L-Lactate by the lldPRD regulatory region. We have also designed other parts which produce various higher and lower concentrations of lldD by varying the strength of the promoter and RBS controlling its production.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1203
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 640
Characterization
Due to shortage of time we could not characterize this part.