Difference between revisions of "Part:BBa K2996501"
 
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<center><img style="max-width: 80%" src="https://2019.igem.org/wiki/images/f/f3/T--sjtu-biox-shanghai--jsf-dbdfull.png" alt="" /></center>
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<center><b>Figure 1.</b> <i>PCR product of DBD with Flag tag(421bp). The ladder represents the size of DNA in bp.</i> </center>
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<center><b>Figure 1.</b> <i>Fig.2 PCR product of DBD with rrnB T1 terminator downstream.(Lane1:product of DBD;Lane 2:2000bp ladder).</i> </center>

Latest revision as of 23:43, 21 October 2019

Terminator put downstream of zinc finger

It is composite part composed of BBa_K2996500 and BBa_B0010.This is used as a half of transcription unit.We aimed to combine part BBa_K2996502 and this part to make an entire production module of C-luciferase-DBD fusion protein. We used PCR to construct this part.As for the terminator,we used primers to bring it to part BBa_K2996500.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 304
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Figure 1. Fig.2 PCR product of DBD with rrnB T1 terminator downstream.(Lane1:product of DBD;Lane 2:2000bp ladder).