Difference between revisions of "Part:BBa K3187018"

Revision as of 14:53, 21 October 2019 (view source) JonathanFu (Talk | contribs) ← Older edit		Latest revision as of 17:51, 21 October 2019 (view source) Malte (Talk | contribs)
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	The enzyme Sortase A is able to form a peptide bond between a C-terminal LPXTG motif <a href="https://parts.igem.org/Part:BBa_K3187019">		The enzyme Sortase A is able to form a peptide bond between a C-terminal LPXTG motif <a href="https://parts.igem.org/Part:BBa_K3187019">
−	(BBa_K3187019)</a> and a N-terminal poly glycine motif	+	(BBa_K3187019)</a> and a N-terminal poly glycine motif (<a href="https://parts.igem.org/Part:BBa_K3187018"target="_blank">BBa_K3187018</a>)
	<sup id="cite_ref-1” class=”reference">		<sup id="cite_ref-1” class=”reference">
	<a href="#cite_note-1">[1]		<a href="#cite_note-1">[1]
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−	. The sequence below is coding for four glycines that can be used as the second recognition motif in a Sortase reaction.	+	. The sequence below is coding for four glycines that can be used as the second recognition motif in a sortase-mediated reaction. For more results visit <a
		+	href="https://parts.igem.org/Part:BBa_K3187028"
		+	target="_blank">BBa_K3187028</a>
	</p>		</p>
		+

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Latest revision as of 17:51, 21 October 2019

PolyG Tag (GGGG) for Sortase-mediated Ligation

Profile

Name	PolyG-tag
Base pairs	12
Molecular weight	300 Da
Origin	Synthetic

Usage and Biology

The enzyme Sortase A is able to form a peptide bond between a C-terminal LPXTG motif (BBa_K3187019) and a N-terminal poly glycine motif (BBa_K3187018) ^{[1] [2] [3]} . The sequence below is coding for four glycines that can be used as the second recognition motif in a sortase-mediated reaction. For more results visit BBa_K3187028

References

1. ↑ Tsukiji, S. and Nagamune, T. (2009) Sortase-Mediated Ligation: A Gift from Gram-Positive Bacteria to Protein Engineering [1]
2. ↑ Proft, T. (2010) Sortase-mediated protein ligation: an emerging biotechnology tool for protein modification and immobilisation [2]
3. ↑ Mao, H., Hart, S. A., Schink, A., and Pollok, B. A. (2004) Sortase-mediated protein ligation: a new method for protein engineering [3]

Sequence and Features