Difference between revisions of "Part:BBa K2982006"
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A forward primer that contains RBS34 which is 60 base pairs long that can be used to amplify the GFP part BBa_E0040 by performing PCR. It can be used to characterise the RBS activity easily by measuring its fluorescence. | A forward primer that contains RBS34 which is 60 base pairs long that can be used to amplify the GFP part BBa_E0040 by performing PCR. It can be used to characterise the RBS activity easily by measuring its fluorescence. | ||
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This will be used as a standard in measuring RBS activity as RBS strength is often measured relative to RBS34. This primer contains an XbaI restriction enzyme site, a 21-nucleotide random sequence, RBS34, another random sequence for binding, then the first 21 nucleotides of the GFP sequence | This will be used as a standard in measuring RBS activity as RBS strength is often measured relative to RBS34. This primer contains an XbaI restriction enzyme site, a 21-nucleotide random sequence, RBS34, another random sequence for binding, then the first 21 nucleotides of the GFP sequence | ||
Latest revision as of 14:36, 21 October 2019
Forward primer with BBa_B0034 for amplifying BBa_E0040
A forward primer that contains RBS34 which is 60 base pairs long that can be used to amplify the GFP part BBa_E0040 by performing PCR. It can be used to characterise the RBS activity easily by measuring its fluorescence.
This will be used as a standard in measuring RBS activity as RBS strength is often measured relative to RBS34. This primer contains an XbaI restriction enzyme site, a 21-nucleotide random sequence, RBS34, another random sequence for binding, then the first 21 nucleotides of the GFP sequence
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 5
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 5
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 5
- 1000COMPATIBLE WITH RFC[1000]