Difference between revisions of "Part:BBa K3117006:Design"
 
(4 intermediate revisions by 2 users not shown)
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
We considered which leader sequences are most effective to effect secretion of our protein of interest.
+
The Igk leader sequence was codon optimized for CHO cells with respect to the BioBrick standard.
−
 
+
−
 
+
  
 
===Source===
 
===Source===
  
 
This part comes from genomic sequence.
 
This part comes from genomic sequence.
−
 
−
===References===
 
−
Feng, Y., Gong, R., & Dimitrov, D. S. (2011). Design, expression and characterization of a soluble single-chain functional human neonatal Fc receptor. Protein expression and purification, 79(1), 66-71.
 

Latest revision as of 00:17, 22 October 2019


Igk leader sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The Igk leader sequence was codon optimized for CHO cells with respect to the BioBrick standard.

Source

This part comes from genomic sequence.