Difference between revisions of "Part:BBa K2999001"
 
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<partinfo>BBa_K2999001 short</partinfo>
 
<partinfo>BBa_K2999001 short</partinfo>
  
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This part is mainly used for the extracellular polysaccharide hydrolase PslG encoding ''Pseudomonas aeruginosa'' PAO1 biofilm and the hydrolysis of such extracellular polysaccharides. The first 50 bp of the sequence is its ribosome binding site.
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This part is mainly used to encode the extracellular polysaccharide hydrolase PslG and the hydrolysis of such extracellular polysaccharides of biofilm produced by ''Pseudomonas aeruginosa'' PAO1 . The first 50 bp of the sequence is its ribosome binding site.
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For compatibility, 254<sup>th<sup><br/> nucleotide G was changed to A (same as leucine) and 1358 nucleotide G to A (same as leucine).
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For compatibility, 254<sup>th</sup> nucleotide G was changed to A (same as leucine) and 1358<sup>th</sup> nucleotide G to A (same as leucine).
  
 
===Usage and Biology===
 
===Usage and Biology===

Latest revision as of 14:32, 21 October 2019


RBS+PslG

This part is mainly used to encode the extracellular polysaccharide hydrolase PslG and the hydrolysis of such extracellular polysaccharides of biofilm produced by Pseudomonas aeruginosa PAO1 . The first 50 bp of the sequence is its ribosome binding site. For compatibility, 254th nucleotide G was changed to A (same as leucine) and 1358th nucleotide G to A (same as leucine).

Usage and Biology

Please click:BBa_K2999009


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 356
    Illegal XhoI site found at 360
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 660
    Illegal NgoMIV site found at 849
    Illegal NgoMIV site found at 857
  • 1000
    COMPATIBLE WITH RFC[1000]