Difference between revisions of "Part:BBa K2974310:Experience"
 
(3 intermediate revisions by the same user not shown)
Line 1: Line 1:
  
 
__NOTOC__
 
__NOTOC__
−
The results for the toehold were confirmed through a dual plasmid transformation in BL21 <i>E. coli.</i> The dual plasmid transformation presented greater fluorescence measured in lux values in comparison to the single switch transformation, confirming the function of the toehold. In addition, sequencing analysis results shown below also confirms the presence of the toehold in comparison to the toehold sequences in the reference paper (Green et al. 2014). The biobricked toehold's (BBa_K2974316) function has been validated through experimentation and sequencing.  
+
The results for the toehold were confirmed through a dual plasmid transformation in BL21 <i>E. coli.</i> The dual plasmid transformation presented greater fluorescence measured in lux values in comparison to the single switch transformation, confirming the function of the toehold. The dual plasmid transformation would not succeed without the correct <i>C. elegans</i> toehold intermediate, so the function of this part (BBa_K2974310) has been validated through experimentation and sequencing (see BBa_K2974316 for sequencing confirmation).  
 
<br>
 
<br>
 
<br>
 
<br>
−
<center>[[File:T--Lambert GA--FLUOROCHART.png|300px]]</center>
+
<center>[[File:T--Lambert GA--FLUOROCHART.png|500px]]</center>
 
<br>
 
<br>
−
<center><I>Figure 1: This graph compares the mean lux values of the <i>C. elegans</i> toehold switch, trigger, and the dual plasmid. The toehold switch and trigger have very similar lux values to LB and plain cells, whereas the dual plasmid has a higher lux value, indicating fluorescence.</I></center>
+
<center><I>Figure 1: This graph compares the mean lux values of the C. elegans toehold switch, trigger, and the dual plasmid. The toehold switch and trigger have very similar lux values to LB and plain cells, whereas the dual plasmid has a higher lux value, indicating fluorescence.</I></center>
  
 
===Applications of BBa_K2974310===
 
===Applications of BBa_K2974310===
−
This sequence is to be used as a proof of concept
+
This sequence is to be used as a proof of concept, and can be used to develop biosensor control devices.
  
 
===User Reviews===
 
===User Reviews===

Latest revision as of 13:48, 21 October 2019


The results for the toehold were confirmed through a dual plasmid transformation in BL21 E. coli. The dual plasmid transformation presented greater fluorescence measured in lux values in comparison to the single switch transformation, confirming the function of the toehold. The dual plasmid transformation would not succeed without the correct C. elegans toehold intermediate, so the function of this part (BBa_K2974310) has been validated through experimentation and sequencing (see BBa_K2974316 for sequencing confirmation).

T--Lambert GA--FLUOROCHART.png


Figure 1: This graph compares the mean lux values of the C. elegans toehold switch, trigger, and the dual plasmid. The toehold switch and trigger have very similar lux values to LB and plain cells, whereas the dual plasmid has a higher lux value, indicating fluorescence.

Applications of BBa_K2974310

This sequence is to be used as a proof of concept, and can be used to develop biosensor control devices.

User Reviews

UNIQa74d52af0f091fd6-partinfo-00000000-QINU UNIQa74d52af0f091fd6-partinfo-00000001-QINU