Difference between revisions of "Part:BBa K2992011"

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===Usage and Biology===
 
===Usage and Biology===
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This part entry represents the RBS from the alternative sigma factor-encoding gene <i>botR</i>. BotR is a positive regulator of neurotoxin production. In our project we use the <i>botR</i> RBS as part of the whole regulatory region for the expression of <i>botR</i> comprising P<i>botR</i> and its native 5'-UTR containing the RBS ([https://parts.igem.org/Part:BBa_K2992014 BBa_K2992014]). This entry, the RBS was also used in conjunction with <i>botR</i> lacking its native promoter to allow for polar expression from the <i>pyrD</i> and <i>pyrE</i> genes on the <i>C. sporogenes</i> genome following genomic integration of <i>botR</i> . <br><br>
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This part entry represents the RBS from the alternative sigma factor-encoding gene <i>botR</i>. BotR is a positive regulator of neurotoxin production. In our project we use the <i>botR</i> RBS as part of the whole regulatory region for the expression of <i>botR</i> comprising P<i>botR</i> and its native 5'-UTR containing the RBS ([https://parts.igem.org/Part:BBa_K2992014 BBa_K2992014]). This part was also used in conjunction with <i>botR</i> lacking its native promoter to allow for polar expression from the <i>pyrDKE</i> operon on the <i>C. sporogenes</i> genome, following genomic integration of the respective <i>botR</i>-expression module . <br><br>
  
 
===Characterisation===
 
===Characterisation===

Latest revision as of 00:59, 22 October 2019


RBS region for botR gene from C. botulinum

RBS region for the botR gene in C. botulinum


Usage and Biology

This part entry represents the RBS from the alternative sigma factor-encoding gene botR. BotR is a positive regulator of neurotoxin production. In our project we use the botR RBS as part of the whole regulatory region for the expression of botR comprising PbotR and its native 5'-UTR containing the RBS (BBa_K2992014). This part was also used in conjunction with botR lacking its native promoter to allow for polar expression from the pyrDKE operon on the C. sporogenes genome, following genomic integration of the respective botR-expression module .

Characterisation

Data incoming

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

Raffestin, S., Dupuy, B., Marvaud, J. and Popoff, M. (2004). BotR/A and TetR are alternative RNA polymerase sigma factors controlling the expression of the neurotoxin and associated protein genes in Clostridium botulinum type A and Clostridium tetani. Molecular Microbiology, 55(1), pp.235-249.

Zhang, Z., Korkeala, H., Dahlsten, E., Sahala, E., Heap, J., Minton, N. and Lindström, M. (2013). Two-Component Signal Transduction System CBO0787/CBO0786 Represses Transcription from Botulinum Neurotoxin Promoters in Clostridium botulinum ATCC 3502. PLoS Pathogens, 9(3), p.e1003252.