Difference between revisions of "Part:BBa K108020"
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DNA adenine methylase of E.coli
 
DNA adenine methylase of E.coli
  
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===Information contributed by City of London UK (2021)===
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===Usage and Biology===
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Part information is collated here to help future users of the BioBrick registry.
  
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Metadata:
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<span class='h3bb'>Sequence and Features</span>
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*'''Group:''' City of London UK 2021
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<partinfo>BBa_K108020 SequenceAndFeatures</partinfo>
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*'''Author:''' Lucas Ng
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*'''Summary:''' Added information collated from existing scientific studies
  
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dam causes DNA methylation within the sequence GATC, preventing cleavage by the MboI restriction endonuclease
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===Functional Parameters===
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<ref>Geier, G. E., and P. Modrich. 1979. “Recognition Sequence of the Dam Methylase of Escherichia Coli K12 and Mode of Cleavage of Dpn I Endonuclease.” The Journal of Biological Chemistry 254 (4): 1408–13. https://pubmed.ncbi.nlm.nih.gov/368070.</ref>.
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<partinfo>BBa_K108020 parameters</partinfo>
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It is likely to act in postreplication mismatch repair
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<ref>Lu, A. L., S. Clark, and P. Modrich. 1983. “Methyl-Directed Repair of DNA Base-Pair Mismatches in Vitro.” Proceedings of the National Academy of Sciences 80 (15): 4639–43. https://doi.org/10.1073/pnas.80.15.4639.</ref>
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rather than as a member of a restriction modification system, despite sharing sequence specificity with several type II restriction methylases and endonucleases as part of the N(4)/N(6)-methyltransferase family
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<ref>Horton, John R., Xing Zhang, Robert M. Blumenthal, and Xiaodong Cheng. 2015. “Structures of Escherichia Coli DNA Adenine Methyltransferase (Dam) in Complex with a Non-GATC Sequence: Potential Implications for Methylation-Independent Transcriptional Repression.” Nucleic Acids Research 43 (8): 4296–4308. https://doi.org/10.1093/nar/gkv251.</ref>.
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Furthermore, it may also be involved in DNA replication
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<ref>Smith, D. W., A. M. Garland, G. Herman, R. E. Enns, T. A. Baker, and J. W. Zyskind. 1985. “Importance of State of Methylation of OriC GATC Sites in Initiation of DNA Replication in Escherichia Coli.” The EMBO Journal 4 (5): 1319–26. https://pubmed.ncbi.nlm.nih.gov/3891329.</ref>
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====Reaction====
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a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an N6-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-homocysteine
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<ref>“Rhea - Annotated Reactions Database.” n.d. Rhea. Accessed July 26, 2021. https://www.rhea-db.org/rhea/15197.</ref>
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===References===

Latest revision as of 17:36, 26 July 2021

Dam

DNA adenine methylase of E.coli

Information contributed by City of London UK (2021)

Part information is collated here to help future users of the BioBrick registry.

Metadata:

  • Group: City of London UK 2021
  • Author: Lucas Ng
  • Summary: Added information collated from existing scientific studies

dam causes DNA methylation within the sequence GATC, preventing cleavage by the MboI restriction endonuclease [1].

It is likely to act in postreplication mismatch repair [2] rather than as a member of a restriction modification system, despite sharing sequence specificity with several type II restriction methylases and endonucleases as part of the N(4)/N(6)-methyltransferase family [3].

Furthermore, it may also be involved in DNA replication [4]

Reaction

a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an N6-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-homocysteine [5]

References

  1. ↑ Geier, G. E., and P. Modrich. 1979. “Recognition Sequence of the Dam Methylase of Escherichia Coli K12 and Mode of Cleavage of Dpn I Endonuclease.” The Journal of Biological Chemistry 254 (4): 1408–13. https://pubmed.ncbi.nlm.nih.gov/368070.
  2. ↑ Lu, A. L., S. Clark, and P. Modrich. 1983. “Methyl-Directed Repair of DNA Base-Pair Mismatches in Vitro.” Proceedings of the National Academy of Sciences 80 (15): 4639–43. https://doi.org/10.1073/pnas.80.15.4639.
  3. ↑ Horton, John R., Xing Zhang, Robert M. Blumenthal, and Xiaodong Cheng. 2015. “Structures of Escherichia Coli DNA Adenine Methyltransferase (Dam) in Complex with a Non-GATC Sequence: Potential Implications for Methylation-Independent Transcriptional Repression.” Nucleic Acids Research 43 (8): 4296–4308. https://doi.org/10.1093/nar/gkv251.
  4. ↑ Smith, D. W., A. M. Garland, G. Herman, R. E. Enns, T. A. Baker, and J. W. Zyskind. 1985. “Importance of State of Methylation of OriC GATC Sites in Initiation of DNA Replication in Escherichia Coli.” The EMBO Journal 4 (5): 1319–26. https://pubmed.ncbi.nlm.nih.gov/3891329.
  5. ↑ “Rhea - Annotated Reactions Database.” n.d. Rhea. Accessed July 26, 2021. https://www.rhea-db.org/rhea/15197.