Difference between revisions of "Part:BBa K3046033:Design"
| Line 14: | Line 14: | ||
===Source=== | ===Source=== | ||
| − | Our IS2 parts were created by Dorthe Holm | + | Our IS2 parts were created by Dorthe Holm in 2013 as part of a ph.d. thesis [1] |
===References=== | ===References=== | ||
| + | [1] Holm, D. K. (2013). Development and Implementation of Novel Genetic Tools for Investigation of Fungal Secondary Metabolism. Kgs. Lyngby: Technical University of Denmark. | ||
Latest revision as of 15:50, 21 October 2019
IS2 downstream sequence for A. niger genome integration
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 118
Illegal PstI site found at 1262
Illegal PstI site found at 1863 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 118
Illegal PstI site found at 1262
Illegal PstI site found at 1863 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 118
Illegal BglII site found at 1360 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 118
Illegal PstI site found at 1262
Illegal PstI site found at 1863 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 118
Illegal PstI site found at 1262
Illegal PstI site found at 1863
Illegal NgoMIV site found at 1250 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 1751
Illegal SapI site found at 2060
Design Notes
This part is not RFC[1000] compatible, and is therefore best used with cloning methods that does not rely on either 3A or TypeIIS assembly. DTU-Denmark 2019 has used Gibson assembly for this part with great success. It should be possible to de novo synthesize this part. Generally the part does not contain too many repeats, and we have had no problems with designing primers for PCR/sequencing that binds in this sequence.
Source
Our IS2 parts were created by Dorthe Holm in 2013 as part of a ph.d. thesis [1]
References
[1] Holm, D. K. (2013). Development and Implementation of Novel Genetic Tools for Investigation of Fungal Secondary Metabolism. Kgs. Lyngby: Technical University of Denmark.