Difference between revisions of "Part:BBa K2924033:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | Expression system for the expression and secretion of the protein in B. subtilis | + | Expression system for the expression and secretion of the protein in <i>B. subtilis</i> |
===Source=== | ===Source=== | ||
| − | PBSMul1 plasmid was kindly provided by Dr. Andreas Knapp from the research center Jülich. The DNA sequence was acquired by reverse-translating the protein sequence of a-s1-casein. a-s1-casein was commercial synthesized by GenScript. | + | PBSMul1-SPNprE plasmid was kindly provided by Dr. Andreas Knapp from the research center Jülich. The DNA sequence was acquired by reverse-translating the protein sequence of a-s1-casein. a-s1-casein was commercial synthesized by GenScript. |
===References=== | ===References=== | ||
Latest revision as of 11:48, 21 October 2019
Hpall + SPNprE + α-s1-casein + fd terminator
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1100
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Expression system for the expression and secretion of the protein in B. subtilis
Source
PBSMul1-SPNprE plasmid was kindly provided by Dr. Andreas Knapp from the research center Jülich. The DNA sequence was acquired by reverse-translating the protein sequence of a-s1-casein. a-s1-casein was commercial synthesized by GenScript.
References
Brockmeier, U., et al. (2006). "Systematic screening of all signal peptides from Bacillus subtilis: a powerful strategy in optimizing heterologous protein secretion in Gram-positive bacteria." J Mol Biol 362(3): 393-402.
Knapp, A., et al. (2017). "Activity-independent screening of secreted proteins using split GFP." J Biotechnol 258: 110-116.