Difference between revisions of "Part:BBa K3173007"
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Linker for Tango like GPCR assays. | Linker for Tango like GPCR assays. | ||
Couple it to GPCR´s for improved arrestin binding plus mediated TEV Protease cleavage to release downstream POI. | Couple it to GPCR´s for improved arrestin binding plus mediated TEV Protease cleavage to release downstream POI. | ||
| − | Before coupling upstream GPCR should be checked for arrestin recruitment and/or typical Phosphorylation motifs to allow such recruitment. | + | Before coupling, upstream GPCR should be checked for arrestin recruitment and/or typical Phosphorylation motifs to allow such recruitment. See BBa_K3173010 for a possible composite construct design. |
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Latest revision as of 14:27, 21 October 2019
v2-tail with efficient TCS and SV 40 NLS
Linker for Tango like GPCR assays. Couple it to GPCR´s for improved arrestin binding plus mediated TEV Protease cleavage to release downstream POI. Before coupling, upstream GPCR should be checked for arrestin recruitment and/or typical Phosphorylation motifs to allow such recruitment. See BBa_K3173010 for a possible composite construct design.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]