Difference between revisions of "Part:BBa K3031015:Design"
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"https://2019.igem.org/wiki/images/9/9a/T--SUIS_Shanghai--ironQSassay.png"  
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IronQS2 with the FUR box located at -10 position performed well at suppressing genes in low iron environments (in presence of iron chelator 2-2' Bipyridine)
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<h3>IronQS2 with the FUR box located at -10 position performed well at suppressing genes in low iron environments (in presence of iron chelator 2-2' Bipyridine)</h3>
 
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===Source===
 
===Source===
  
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BioBrick sequences.  
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The part sequences were all sourced from the registry. We did make a new part which was simply the insertion of the FUR box (part BBa_K) into the -10 sequence of the luxI promoter.
  
 
===References===
 
===References===

Latest revision as of 10:44, 21 October 2019


LuxI with FUR box located in the -10 region


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Insertion of the FUR box was informed by the report by Chu et al., (2015). This study inserted the FUR box region at different locations on a plasmid containing the LuxR/LuxI QS system. The construct with the insert at the -10 region (termed ironQS2) performed the best in experiments testing different constructs (as seen below) in low iron environments.

IronQS2 with the FUR box located at -10 position performed well at suppressing genes in low iron environments (in presence of iron chelator 2-2' Bipyridine)

Source

The part sequences were all sourced from the registry. We did make a new part which was simply the insertion of the FUR box (part BBa_K) into the -10 sequence of the luxI promoter.

References

Chu, Teng, Ni, Chunshan, Zhang, Lingzhi, Wang, Qiyao, Xiao, Jingfan, Zhang, Yuanxing, & Liu, Qin. (2015). A quorum sensing-based in vivo expression system and its application in multivalent bacterial vaccine. Microbial Cell Factories,14(1), 37.

Rai, N., Anand, R., Ramkumar, K., Sreenivasan, V., Dabholkar, S., Venkatesh, K., . . . Buchler, N. (2012). Prediction by Promoter Logic in Bacterial Quorum Sensing (Prediction by Promoter Logic in Quorum Sensing). PLoS Computational Biology,8(1), E1002361.