Difference between revisions of "Part:BBa K3031015:Design"
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===Design Notes===
 
===Design Notes===
 
Insertion of the FUR box was informed by the report by Chu et al., (2015). This study inserted the FUR box region at different locations on a plasmid containing the LuxR/LuxI QS system. The construct with the insert at the -10 region (termed ironQS2) performed the best in experiments testing different constructs (as seen below) in low iron environments.  
 
Insertion of the FUR box was informed by the report by Chu et al., (2015). This study inserted the FUR box region at different locations on a plasmid containing the LuxR/LuxI QS system. The construct with the insert at the -10 region (termed ironQS2) performed the best in experiments testing different constructs (as seen below) in low iron environments.  
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[[File:T--SUIS Shanghai--ironQSassay.png|frame|center|'''OxyR ([https://parts.igem.org/BBa_K1104200 BBa_K1104200]) ''']]
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<img src="https://2019.igem.org/wiki/images/0/06/T--SUIS_Shanghai--ironQSconstructs.png"|height=300px width=400px>
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<img src="https://2019.igem.org/wiki/images/9/9a/T--SUIS_Shanghai--ironQSassay.png"|height=300px width=400px>
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<h3>IronQS2 with the FUR box located at -10 position performed well at suppressing genes in low iron environments (in presence of iron chelator 2-2' Bipyridine)</h3>
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===Source===
 
===Source===
  
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BioBrick sequences.  
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The part sequences were all sourced from the registry. We did make a new part which was simply the insertion of the FUR box (part BBa_K) into the -10 sequence of the luxI promoter.
  
 
===References===
 
===References===

Latest revision as of 10:44, 21 October 2019


LuxI with FUR box located in the -10 region


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Insertion of the FUR box was informed by the report by Chu et al., (2015). This study inserted the FUR box region at different locations on a plasmid containing the LuxR/LuxI QS system. The construct with the insert at the -10 region (termed ironQS2) performed the best in experiments testing different constructs (as seen below) in low iron environments.

IronQS2 with the FUR box located at -10 position performed well at suppressing genes in low iron environments (in presence of iron chelator 2-2' Bipyridine)

Source

The part sequences were all sourced from the registry. We did make a new part which was simply the insertion of the FUR box (part BBa_K) into the -10 sequence of the luxI promoter.

References

Chu, Teng, Ni, Chunshan, Zhang, Lingzhi, Wang, Qiyao, Xiao, Jingfan, Zhang, Yuanxing, & Liu, Qin. (2015). A quorum sensing-based in vivo expression system and its application in multivalent bacterial vaccine. Microbial Cell Factories,14(1), 37.

Rai, N., Anand, R., Ramkumar, K., Sreenivasan, V., Dabholkar, S., Venkatesh, K., . . . Buchler, N. (2012). Prediction by Promoter Logic in Bacterial Quorum Sensing (Prediction by Promoter Logic in Quorum Sensing). PLoS Computational Biology,8(1), E1002361.