Difference between revisions of "Part:BBa K3170996"
 
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Tobacco etching virus protease, Part of the protease-protease regulation system
 
Tobacco etching virus protease, Part of the protease-protease regulation system
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<ul>
 
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<li>TEV Protease is a recombinant protease derived from Tobacco Etch Virus (TEV) Nla, which is used to excise the affinity tag of the purified fusion protein. TEV protease has strong site specificity and can recognize the seven amino acid sequence of EXXYXQ(G/S) (Glu-Asn-Leu- Tyr-Phe-Gln-Gly). The most common one is ENLYFQG, and its cleavage site is at Between glutamine and glycine or serine.</li>
 
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==Experimental Results==
 
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[[File:T--LZU-CHINA--TEVp.png|600px|thumb|center|]]
 
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<li>Using the specificity of TEVp, we cleave the recognition sites of ALKBH (Fig.A) and Casp3 (Fig.B) linked by the target sequence, which are active.</li>
 
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<!-- Add more about the biology of this part here
 
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Latest revision as of 09:19, 21 October 2019


TEVp

Tobacco etching virus protease, Part of the protease-protease regulation system

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]