Difference between revisions of "Part:BBa K3254027"
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=Usage= | =Usage= | ||
[[File:T--GENAS_China--excision_with_backbone.PNG|200px|thumb|left|The composition and principle of the experimental system]] | [[File:T--GENAS_China--excision_with_backbone.PNG|200px|thumb|left|The composition and principle of the experimental system]] | ||
| − | We placed this part downstreamed a Ptac promoter([[Part:BBa_K2572025|BBa_K2572025]]) on a P15A plasmid, and it can cause the recombinant between its corresponding att sites (see [[Part:BBa_K3254002|BBa_K3254002]]) on a pSB4C5 plasmid when full induced. The information about orthogonality characterization (include experimental setup) can be found on the page of | + | We placed this part downstreamed a Ptac promoter([[Part:BBa_K2572025|BBa_K2572025]]) on a P15A plasmid, and it can cause the recombinant between its corresponding att sites (see [[Part:BBa_K3254002|BBa_K3254002]]) on a pSB4C5 plasmid when full induced. The information about orthogonality characterization (include experimental setup) can be found on the page of Int7 integrase ([[Part:BBa_K3254007|BBa_K3254007]]). |
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Latest revision as of 08:42, 21 October 2019
Translational Unit for Int7 integrase
This part can be placed downstreamed a prmoter. It is sure that when it placed under a Ptac promoter on a P15A plasmid and full induced, the recombination between Int7 attP and attB will happen as expected. The RiboJ(BBa_K2615996) part was added before the RBS-coding sequence, so that the modularity and reuseability would be improved.
Usage
We placed this part downstreamed a Ptac promoter(BBa_K2572025) on a P15A plasmid, and it can cause the recombinant between its corresponding att sites (see BBa_K3254002) on a pSB4C5 plasmid when full induced. The information about orthogonality characterization (include experimental setup) can be found on the page of Int7 integrase (BBa_K3254007).
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 389
Illegal PstI site found at 938 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 80
Illegal PstI site found at 389
Illegal PstI site found at 938 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 268
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 389
Illegal PstI site found at 938 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 389
Illegal PstI site found at 938
Illegal AgeI site found at 929
Illegal AgeI site found at 1330 - 1000COMPATIBLE WITH RFC[1000]