Difference between revisions of "Part:BBa K103020"
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*Fusion partners are connected via Gly-Ser-Gly linker | *Fusion partners are connected via Gly-Ser-Gly linker | ||
*Prey protein should be attached via linker (using BamHI+ Biobrick standard suffix enzyme). | *Prey protein should be attached via linker (using BamHI+ Biobrick standard suffix enzyme). | ||
| − | *Fusion protein can be purified on NiNTA bead. | + | *Fusion protein can be purified on NiNTA bead (thanks to presence of N-teminal His-tag). |
| + | *Generator under control of T7 promoter . | ||
*Interaction between hunter and prey added to the medium makes cells ampicillin resistant | *Interaction between hunter and prey added to the medium makes cells ampicillin resistant | ||
*This part uses our NdeI/SacI/BamHI fusion cloning substandard | *This part uses our NdeI/SacI/BamHI fusion cloning substandard | ||
Latest revision as of 12:55, 28 October 2008
omega_linker under PT7
Usage and Biology
- Construct for creating prey fusions for our 'hunter and prey' selection system
- Fusion partners are connected via Gly-Ser-Gly linker
- Prey protein should be attached via linker (using BamHI+ Biobrick standard suffix enzyme).
- Fusion protein can be purified on NiNTA bead (thanks to presence of N-teminal His-tag).
- Generator under control of T7 promoter .
- Interaction between hunter and prey added to the medium makes cells ampicillin resistant
- This part uses our NdeI/SacI/BamHI fusion cloning substandard
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 356