Difference between revisions of "Part:BBa K3165040"
 
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<partinfo>BBa_K3165040 short</partinfo>
 
<partinfo>BBa_K3165040 short</partinfo>
  
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This part places the fluorescent protein - ECFP under the inducible pCpcG2 promoter.
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This part places the fluorescent protein - ECFP <html><a  href="https://parts.igem.org/Part:BBa_K3165006">(BBa_K3165006)</a></html> under the inducible PcpcG2 promoter <html><a  href="https://parts.igem.org/Part:BBa_K2012015">(BBa_K2012015)</a></html>.
  
  
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===Usage and Biology===
 
===Usage and Biology===
  
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This part encodes for the engineered cyan fluorescent protein (ECFP) under an inducible promoter pCpcG2. The promoter is activated by the phosphorylated form of CcaR (a response regulator) and deactivated by dephosphorylated CcaR. the phosphorylation/dephosphorylation of CcaR depends on the light that is shone and perceived by the trans-membrane CcaS protein.
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This part encodes for the engineered cyan fluorescent protein <html><a  href="https://parts.igem.org/Part:BBa_K3165006">(ECFP)</a></html> under an inducible promoter <html><a  href="https://parts.igem.org/Part:BBa_K2012015">PcpcG2</a></html>. The promoter is activated by the phosphorylated form of CcaR (a response regulator) and deactivated by dephosphorylated CcaR. the phosphorylation/dephosphorylation of CcaR depends on the light that is shone and perceived by the trans-membrane CcaS protein.
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Upon light stimulation, the production of ECFP is triggered leading to a fluorescence by the bacteria. By fluorescence measurements, the functioning of the light-sensitive system can be quantified.
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Upon light stimulation, the production of ECFP is triggered leading to fluorescence by the bacteria. By fluorescence measurements, the functioning of the light-sensitive system can be quantified.
 
This part is used in association with the CcaS-CcaR system as a light-induced system to quantify the protein expression.
 
This part is used in association with the CcaS-CcaR system as a light-induced system to quantify the protein expression.
  

Latest revision as of 18:49, 21 October 2019


ECFP under PcpcG2 expression

This part places the fluorescent protein - ECFP (BBa_K3165006) under the inducible PcpcG2 promoter (BBa_K2012015).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Usage and Biology

This part encodes for the engineered cyan fluorescent protein (ECFP) under an inducible promoter PcpcG2. The promoter is activated by the phosphorylated form of CcaR (a response regulator) and deactivated by dephosphorylated CcaR. the phosphorylation/dephosphorylation of CcaR depends on the light that is shone and perceived by the trans-membrane CcaS protein. Upon light stimulation, the production of ECFP is triggered leading to fluorescence by the bacteria. By fluorescence measurements, the functioning of the light-sensitive system can be quantified. This part is used in association with the CcaS-CcaR system as a light-induced system to quantify the protein expression.