Difference between revisions of "Part:BBa K079014"

 
 
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K079014 short</partinfo>
 
<partinfo>BBa_K079014 short</partinfo>
  
−
This part has been derived from the BBa_J22101 part by PCR. Infact, BBa_J22101 was not working due to a 35 bp insertion upstream of the LacY coding sequence. We amplied the right LacY gene and inserted it into a standard plasmid from the Registry.
+
This part has been derived from the BBa_J22101 part by PCR. Infact, BBa_J22101 was not working due to a 35 bp insertion upstream of the LacY coding sequence. We amplified the right LacY gene and inserted it into a standard plasmid from the Registry.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 14:18, 24 October 2008

LacY transporter protein from E. coli

This part has been derived from the BBa_J22101 part by PCR. Infact, BBa_J22101 was not working due to a 35 bp insertion upstream of the LacY coding sequence. We amplified the right LacY gene and inserted it into a standard plasmid from the Registry.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 757
  • 1000
    COMPATIBLE WITH RFC[1000]