Difference between revisions of "Part:BBa K103016"
 
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<partinfo>BBa_K103016 short</partinfo>
 
<partinfo>BBa_K103016 short</partinfo>
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<!-- Add more about the biology of this part here
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<!-- Add more about the biology of this part here -->
 
===Usage and Biology===
 
===Usage and Biology===
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*It's fusion of E. coli outer membrane protein (OmpA) domain (BBa_K103006) with beta-lactamase subunit omega (BBa_K103012)
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*It's fusion of E. coli outer membrane protein (OmpA) domain ([[Part:BBa_K103006 | BBa_K103006]]) with beta-lactamase subunit omega ([[Part:BBa_K103012 | BBa_K103012]])
 
*Fusion partners are connected via Gly-Ser-Gly linker
 
*Fusion partners are connected via Gly-Ser-Gly linker
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*Part of our 'hunter and prey' selection system
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*It's base for creation of hunter fusions in 'hunter and prey' selection system
 
*Hunter protein should be attached via the second linker (using BamHI+ Biobrick standard suffix enzyme). It will be exported outside the cell and attached to the outer membrane
 
*Hunter protein should be attached via the second linker (using BamHI+ Biobrick standard suffix enzyme). It will be exported outside the cell and attached to the outer membrane
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*Interaction between hunter and prey (fused with BBa_K103019) makes cells ampicillin resistant
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*Interaction between hunter and prey (fused with [[Part:BBa_K103019 | BBa_K103019]]) makes cells ampicillin resistant
 
*This part uses our NdeI/SacI/BamHI fusion cloning substandard
 
*This part uses our NdeI/SacI/BamHI fusion cloning substandard
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| [[Image:Ompalinkomega.jpg|260px]]
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This protein structure was predicted using threading and protein fold prediction and may differ from actual one.
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|}
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Latest revision as of 00:48, 28 October 2008

OmpA_linker_omega_linker



Usage and Biology

  • It's fusion of E. coli outer membrane protein (OmpA) domain ( BBa_K103006) with beta-lactamase subunit omega ( BBa_K103012)
  • Fusion partners are connected via Gly-Ser-Gly linker
  • It's base for creation of hunter fusions in 'hunter and prey' selection system
  • Hunter protein should be attached via the second linker (using BamHI+ Biobrick standard suffix enzyme). It will be exported outside the cell and attached to the outer membrane
  • Interaction between hunter and prey (fused with BBa_K103019) makes cells ampicillin resistant
  • This part uses our NdeI/SacI/BamHI fusion cloning substandard
 Ompalinkomega.jpg

This protein structure was predicted using threading and protein fold prediction and may differ from actual one.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 802
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 596