Difference between revisions of "Part:BBa K103013"
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===Usage and Biology===
 
===Usage and Biology===
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* beta fragment of the split enzyme TEM beta-lactamase
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* omega fragment of the split enzyme TEM &beta;-lactamase
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* beta-lactamase activity can be recovered when halves are brought in close proximity by e.g. fusion to interacting domains [http://www.ncbi.nlm.nih.gov/pubmed/17005637?ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum]
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* &beta;-lactamase activity can be recovered when halves are brought in close proximity by e.g. fusion to interacting domains [http://www.ncbi.nlm.nih.gov/pubmed/17005637?ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum]
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* works with second half of beta lactamase (part BBa_K103009)
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* works with second half of &beta;-lactamase (part [https://parts.igem.org/Part:BBa_K103009 BBa_K103009])
 
* contains linker module that allows proper binding of fusion partners
 
* contains linker module that allows proper binding of fusion partners
 
* This part uses our NdeI/SacI/BamHI fusion cloning substandard
 
* This part uses our NdeI/SacI/BamHI fusion cloning substandard
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| [[Image:Linkermoega.jpg]]
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This protein structure was predicted using threading and protein fold prediction and may differ from actual one.
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Latest revision as of 12:28, 28 October 2008

linker fused to omega fragment of TEM beta-lactamase



Usage and Biology

  • omega fragment of the split enzyme TEM β-lactamase
  • β-lactamase activity can be recovered when halves are brought in close proximity by e.g. fusion to interacting domains [http://www.ncbi.nlm.nih.gov/pubmed/17005637?ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum]
  • works with second half of β-lactamase (part BBa_K103009)
  • contains linker module that allows proper binding of fusion partners
  • This part uses our NdeI/SacI/BamHI fusion cloning substandard
 Linkermoega.jpg

This protein structure was predicted using threading and protein fold prediction and may differ from actual one.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 190