Difference between revisions of "Part:BBa K3059619"
 
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csgBAC operon isolated from the E. coli (MG1655) genome via PCR. Contains 22 basepair upstream region, so an additional RBS is unnecessary when assembling transcriptional units with this part. The native curli operon contains two divergent operons: csgBAC as well as csgDEFG. To create a synthetic curli operon, csgEFG is necessary as well as csgBAC (for example, though csgA constitutes the main subunit of the eventual fiber, csgG is responsible for fiber export out of the cell). csgD regulates csgBAC, and is thus unnecessary in a synthetic operon where both component operons are combined under the control of one promoter.
 
csgBAC operon isolated from the E. coli (MG1655) genome via PCR. Contains 22 basepair upstream region, so an additional RBS is unnecessary when assembling transcriptional units with this part. The native curli operon contains two divergent operons: csgBAC as well as csgDEFG. To create a synthetic curli operon, csgEFG is necessary as well as csgBAC (for example, though csgA constitutes the main subunit of the eventual fiber, csgG is responsible for fiber export out of the cell). csgD regulates csgBAC, and is thus unnecessary in a synthetic operon where both component operons are combined under the control of one promoter.
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<img src="https://2019.igem.org/wiki/images/2/2b/T--William_and_Mary--wildvssynthcurliJ.jpeg" width="800px" class="center"/>
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When successfully assembled in a transcriptional unit with  a promoter, csgEFG, an RBS preceding csgEFG, and a terminator, this part results in curli amyloid fiber production. Curli fibers constitute the main proteinaceous component of Enterobacteriaceae species such as E. coli (Barnhart & Chapman, 2006).
 
When successfully assembled in a transcriptional unit with  a promoter, csgEFG, an RBS preceding csgEFG, and a terminator, this part results in curli amyloid fiber production. Curli fibers constitute the main proteinaceous component of Enterobacteriaceae species such as E. coli (Barnhart & Chapman, 2006).
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<img src="https://2019.igem.org/wiki/images/5/58/T--William_and_Mary--curlioperonTU.jpeg" width="750px" class="center"/>
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Proposed transcriptional unit using this part. For a 3G-compatible version of this part (and information on assembling a complete synthetic curli operon), see BBa_K3059628.
  
 
Though this part contains a non-Biobrick PstI cut site, it lacks BsaI and SapI cutsites and is thus type IIS-compatible and legal.
 
Though this part contains a non-Biobrick PstI cut site, it lacks BsaI and SapI cutsites and is thus type IIS-compatible and legal.
  
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For a 3G-compatible version of this part (and information on assembling a complete synthetic curli operon), see BBa_K3059628.
 
  
 
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<!-- Add more about the biology of this part here

Latest revision as of 23:00, 20 October 2019


csgBAC + 22 bp upstream

csgBAC operon isolated from the E. coli (MG1655) genome via PCR. Contains 22 basepair upstream region, so an additional RBS is unnecessary when assembling transcriptional units with this part. The native curli operon contains two divergent operons: csgBAC as well as csgDEFG. To create a synthetic curli operon, csgEFG is necessary as well as csgBAC (for example, though csgA constitutes the main subunit of the eventual fiber, csgG is responsible for fiber export out of the cell). csgD regulates csgBAC, and is thus unnecessary in a synthetic operon where both component operons are combined under the control of one promoter.

When successfully assembled in a transcriptional unit with a promoter, csgEFG, an RBS preceding csgEFG, and a terminator, this part results in curli amyloid fiber production. Curli fibers constitute the main proteinaceous component of Enterobacteriaceae species such as E. coli (Barnhart & Chapman, 2006).

Proposed transcriptional unit using this part. For a 3G-compatible version of this part (and information on assembling a complete synthetic curli operon), see BBa_K3059628.

Though this part contains a non-Biobrick PstI cut site, it lacks BsaI and SapI cutsites and is thus type IIS-compatible and legal.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 889
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 889
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 889
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 889
  • 1000
    COMPATIBLE WITH RFC[1000]