Difference between revisions of "Part:BBa K1486003:Experience"
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===Applications of BBa_K1486003=== | ===Applications of BBa_K1486003=== | ||
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===User Reviews=== | ===User Reviews=== | ||
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We developed a new part([https://parts.igem.org/Part:BBa_K1777005 BBa_K1777005]) based on this parts.When we planned to use this part to link our fusion component,we found it hard to design a proper primer to amplify it because of mutiple primer binding site as a result of tandem GGGS. Therefore, we first improved it by change the first GGGS to a natural linker obtained from linker database.In addition,the codon is optimized for mammalian, and we insert a NLS to promote locating in cell nucleus. Such an improved GS linker could be easily assembled to your protein via overlap PCR and offer the property of nuclear localization. | We developed a new part([https://parts.igem.org/Part:BBa_K1777005 BBa_K1777005]) based on this parts.When we planned to use this part to link our fusion component,we found it hard to design a proper primer to amplify it because of mutiple primer binding site as a result of tandem GGGS. Therefore, we first improved it by change the first GGGS to a natural linker obtained from linker database.In addition,the codon is optimized for mammalian, and we insert a NLS to promote locating in cell nucleus. Such an improved GS linker could be easily assembled to your protein via overlap PCR and offer the property of nuclear localization. | ||
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| + | The Sao Carlos-Brazil Team has developed a yeast codon-optimized version of this part with size doubled, it can be accessed at https://parts.igem.org/Part:BBa_K3273015 | ||
Latest revision as of 21:25, 20 October 2019
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K1486003
User Reviews
UNIQ4732d497e7ba41d0-partinfo-00000000-QINU UNIQ4732d497e7ba41d0-partinfo-00000001-QINU UNIQ4732d497e7ba41d0-partinfo-00000002-QINU
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Fudan iGEM 2015 |
We developed a new part(BBa_K1777005) based on this parts.When we planned to use this part to link our fusion component,we found it hard to design a proper primer to amplify it because of mutiple primer binding site as a result of tandem GGGS. Therefore, we first improved it by change the first GGGS to a natural linker obtained from linker database.In addition,the codon is optimized for mammalian, and we insert a NLS to promote locating in cell nucleus. Such an improved GS linker could be easily assembled to your protein via overlap PCR and offer the property of nuclear localization. |
The Sao Carlos-Brazil Team has developed a yeast codon-optimized version of this part with size doubled, it can be accessed at https://parts.igem.org/Part:BBa_K3273015