Difference between revisions of "Part:BBa K2978202"
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<partinfo>BBa_K2978202 short</partinfo> | <partinfo>BBa_K2978202 short</partinfo> | ||
| − | This part is the negative control for the efficiency terminators assay, | + | This part is the negative control for the efficiency terminators assay, when we tested three different sequences for <i>Lactobacillus spp</i> [[Part:BBa_K2978201| K2978201]] [[Part:BBa_K2978202| K2978202]] [[Part:BBa_K2978203| K2978203]] [[Part:BBa_K2978204| K2978204]] and [[Part:BBa_K2978205| K2978205]]. |
The composite part is based in constitutively express a GFP before of each terminator tested, and a RFP in a polycistronic way after the terminator. By comparing the fluorescence of each protein, the efficiency of the terminator could be determined. | The composite part is based in constitutively express a GFP before of each terminator tested, and a RFP in a polycistronic way after the terminator. By comparing the fluorescence of each protein, the efficiency of the terminator could be determined. | ||
| − | This specific terminator is descripted in the next link | + | This specific terminator is descripted in the next link: |
| + | https://parts.igem.org/Part:BBa_B0015 | ||
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Latest revision as of 18:12, 13 December 2019
B0015 Terminators Measurement in Lactobacillus spp
This part is the negative control for the efficiency terminators assay, when we tested three different sequences for Lactobacillus spp K2978201 K2978202 K2978203 K2978204 and K2978205.
The composite part is based in constitutively express a GFP before of each terminator tested, and a RFP in a polycistronic way after the terminator. By comparing the fluorescence of each protein, the efficiency of the terminator could be determined.
This specific terminator is descripted in the next link: https://parts.igem.org/Part:BBa_B0015
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]