Difference between revisions of "Part:BBa K3081104"
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This part is composed of a 42-nt-long dCas9 handle hairpin for Cas9 protein binding and a 40-nt-long transcription terminator hairpin derived from S. pyogenes. When designing a sgRNA for CRISPR/Cas9 system, you just need to put the target sequence before the sgRNA scaffold. | This part is composed of a 42-nt-long dCas9 handle hairpin for Cas9 protein binding and a 40-nt-long transcription terminator hairpin derived from S. pyogenes. When designing a sgRNA for CRISPR/Cas9 system, you just need to put the target sequence before the sgRNA scaffold. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
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| + | == iGEM23_SCUT-China == | ||
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| + | In 2023, SCUT-China successfully applied the CRISPRi system containing this scarfold in a novel strain of Streptomyces sp. SCUT-3 and tested it effective. Details of the usage can be found in BBa_K4621080. | ||
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Latest revision as of 12:16, 9 October 2023
scaffold of sgRNA in CRISPR/Cas system
This part is composed of a 42-nt-long dCas9 handle hairpin for Cas9 protein binding and a 40-nt-long transcription terminator hairpin derived from S. pyogenes. When designing a sgRNA for CRISPR/Cas9 system, you just need to put the target sequence before the sgRNA scaffold.
Usage and Biology
iGEM23_SCUT-China
In 2023, SCUT-China successfully applied the CRISPRi system containing this scarfold in a novel strain of Streptomyces sp. SCUT-3 and tested it effective. Details of the usage can be found in BBa_K4621080.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]