Difference between revisions of "Part:BBa K3037004"
(5 intermediate revisions by 3 users not shown) | |||
Line 20: | Line 20: | ||
− | + | == Overview == | |
− | The TU Dresden team 2019 used this linker | + | The TU Dresden team 2019 used this linker to make the fusion proteins [https://parts.igem.org/Part:BBa_K3037005 (BBa_K3037005)] and [https://parts.igem.org/Part:BBa_K3037003 (BBa_K3037003)]. |
− | This | + | == Characterization == |
+ | This part, encodes for a linker used in between translationally fused proteins. The amino acid composition is "GSAGSAAGSG". The high amounts of glycine and serine residues make it a very flexible linker. | ||
− | + | == Sequence == | |
− | + | This BioBrick is flanked by the RFC25 standard. | |
<partinfo>BBa_K3037004 SequenceAndFeatures</partinfo> | <partinfo>BBa_K3037004 SequenceAndFeatures</partinfo> |
Latest revision as of 22:23, 21 October 2019
Linker
Linker | |
---|---|
Function | Linker for fusion proteins |
Use in | Escherichia coli |
RFC standard | Freiburg RFC25 standard |
Backbone | pSB1C3 |
Submitted by | Team: TU_Dresden 2019 |
Overview
The TU Dresden team 2019 used this linker to make the fusion proteins (BBa_K3037005) and (BBa_K3037003).
Characterization
This part, encodes for a linker used in between translationally fused proteins. The amino acid composition is "GSAGSAAGSG". The high amounts of glycine and serine residues make it a very flexible linker.
Sequence
This BioBrick is flanked by the RFC25 standard.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]