Difference between revisions of "Part:BBa K2922026"
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<partinfo>BBa_K2922026 short</partinfo> | <partinfo>BBa_K2922026 short</partinfo> | ||
===Summary=== | ===Summary=== | ||
| − | Abbreviated as Ekil, lysis protein for Colicin-E1 is a 4.8 kDa protein, which is encoded by gene <i>kil</i>. The lysis protein is required for both Colicin-E1 release and partial cell lysis. <ref>[1]http:// | + | Abbreviated as Ekil, lysis protein for Colicin-E1 is a 4.8 kDa protein, which is encoded by gene <i>kil</i>. The lysis protein is required for both Colicin-E1 release and partial cell lysis. <ref>[1]http://https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1847374/</ref> (Fig.1) |
<table><tr><th>[[File:E1design.png|thumb|720px|Fig.1 Ekil helps Colicin-E1 release and makes partial cell lysis.]]</th><th></table> | <table><tr><th>[[File:E1design.png|thumb|720px|Fig.1 Ekil helps Colicin-E1 release and makes partial cell lysis.]]</th><th></table> | ||
===Identification=== | ===Identification=== | ||
Latest revision as of 17:52, 20 October 2019
Lysis protein for colicin-E1 coding region
Summary
Abbreviated as Ekil, lysis protein for Colicin-E1 is a 4.8 kDa protein, which is encoded by gene kil. The lysis protein is required for both Colicin-E1 release and partial cell lysis. [1] (Fig.1)
Identification
When we were building this circuit, we did the nucleic acid gel electrophoresis experiment to verify. After the circuit was built, we sent the plasmid to sequence, and got the correct sequencing. After new molecular cloning experiments, we did Enzyme-Cut identification to certify the plasmid was correct. We used the XbaI and PstI to cut the plasmid, then we got the target separate fragment-138bp. (Fig.2)
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 118
- 1000COMPATIBLE WITH RFC[1000]