Difference between revisions of "Part:BBa K2973015"
 
(2 intermediate revisions by the same user not shown)
Line 3: Line 3:
 
<partinfo>BBa_K2973015 short</partinfo>
 
<partinfo>BBa_K2973015 short</partinfo>
  
−
Hepatitis B is a DNA virus that attacks the liver and can cause both acute and chronic disease.  
+
<i>Hepatitis B</i> is a DNA virus that attacks the liver and can cause both acute and chronic diseases.  
−
This part represents a small fragment of the virus' genome. It is a small DNA fragment(280 bp), that does not encode for any functional protein or toxin. It's a small part of a sequence that encodes for a DNA polymerase of the virus and in alternative reading frames it encodes for envelope proteins of the virus, or for a specific surface antigen.  
+
This part represents a small fragment of the virus's genome. It is a small DNA fragment(280 bp), that does not encode for any functional protein or toxin. It's a small part of a sequence that encodes for a DNA polymerase of the virus and in alternative reading frames it encodes for envelope proteins of the virus, or for a specific surface antigen.  
  
 
===Usage and Biology===
 
===Usage and Biology===
−
This part serves as a DNA template for amplification reactions (biomarker). In our project, we successfuly amplified a specific region of the part using PCR and Recombinase Polymerase Amplification (RPA).
+
This part serves as a DNA template for amplification reactions (biomarker). In our project, we successfully amplified a specific region of the part using PCR and Recombinase Polymerase Amplification (RPA).
  
−
PCR Reaction
+
PCR Reaction:
  
 
<html>
 
<html>
Line 22: Line 22:
 
</html>
 
</html>
  
−
RPA reaction after cleanup of the amplified product
+
 
 +
RPA reaction after cleanup of the amplified product:
  
 
<html>
 
<html>
Line 34: Line 35:
 
   </body>
 
   </body>
 
</html>
 
</html>
 +
 
<!-- -->
 
<!-- -->
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Latest revision as of 12:58, 21 October 2019


Hepatitis B Virus fragment (281-560bp)

Hepatitis B is a DNA virus that attacks the liver and can cause both acute and chronic diseases. This part represents a small fragment of the virus's genome. It is a small DNA fragment(280 bp), that does not encode for any functional protein or toxin. It's a small part of a sequence that encodes for a DNA polymerase of the virus and in alternative reading frames it encodes for envelope proteins of the virus, or for a specific surface antigen.

Usage and Biology

This part serves as a DNA template for amplification reactions (biomarker). In our project, we successfully amplified a specific region of the part using PCR and Recombinase Polymerase Amplification (RPA).

PCR Reaction:

HTML img Tag


RPA reaction after cleanup of the amplified product:

HTML img Tag

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 210
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]