Difference between revisions of "Part:BBa K3320018"
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<partinfo>BBa_K3320018 short</partinfo> | <partinfo>BBa_K3320018 short</partinfo> | ||
− | Second part of the hypoxia repressor unit from our AND Gate | + | Second part of the hypoxia repressor unit from our AND Gate. |
− | + | <!-- Add more about the biology of this part here <!-- --> | |
− | <!-- Add more about the biology of this part here | + | |
===Usage and Biology=== | ===Usage and Biology=== | ||
+ | It’s an orthogonal repressed promoter by the SrpR protein. In our circuit, this part constitutively produces TetR, which, in our circuit, represses Tet promoter in the absence of hypoxia. | ||
− | |||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K3320018 SequenceAndFeatures</partinfo> | <partinfo>BBa_K3320018 SequenceAndFeatures</partinfo> |
Latest revision as of 17:00, 21 October 2019
pSrp + TetR
Second part of the hypoxia repressor unit from our AND Gate.
Usage and Biology
It’s an orthogonal repressed promoter by the SrpR protein. In our circuit, this part constitutively produces TetR, which, in our circuit, represses Tet promoter in the absence of hypoxia.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 20
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]