Difference between revisions of "Part:BBa K3320015"
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<partinfo>BBa_K3320015 short</partinfo> | <partinfo>BBa_K3320015 short</partinfo> | ||
| − | + | Second part of the lactate repressor unit from our AND Gate. Constitutive production of Lactate Repressor. | |
| − | <!-- Add more about the biology of this part here | + | <!-- Add more about the biology of this part here <!-- --> |
===Usage and Biology=== | ===Usage and Biology=== | ||
| + | This part produces constitutively the LldR, which represses the promoter from BBa_K3320014 in absence of lactate. LldR binds to two operator sites, O1 and O2, with O1 being filled at a lower concentration of LldR. Binding of L-lactate to LldR promotes a conformational change that may disrupt the DNA loop, allowing the formation of the transcription open complex. | ||
| − | |||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K3320015 SequenceAndFeatures</partinfo> | <partinfo>BBa_K3320015 SequenceAndFeatures</partinfo> | ||
Latest revision as of 23:56, 20 October 2019
J23100 + lldR
Second part of the lactate repressor unit from our AND Gate. Constitutive production of Lactate Repressor.
Usage and Biology
This part produces constitutively the LldR, which represses the promoter from BBa_K3320014 in absence of lactate. LldR binds to two operator sites, O1 and O2, with O1 being filled at a lower concentration of LldR. Binding of L-lactate to LldR promotes a conformational change that may disrupt the DNA loop, allowing the formation of the transcription open complex.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 614
- 1000COMPATIBLE WITH RFC[1000]