Difference between revisions of "Part:BBa K3320013"
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This is GFPmut3 based on BBa_E0040 with a secretion tag from the alpha-hemolysin system. | This is GFPmut3 based on BBa_E0040 with a secretion tag from the alpha-hemolysin system. | ||
| − | <!-- Add more about the biology of this part here | + | <!-- Add more about the biology of this part here <!-- --> |
===Usage and Biology=== | ===Usage and Biology=== | ||
| + | GFP is a fluorescent protein from Aequorea victoria. By fusing the HlyA sequence, a peptide consisting of 60 amino acids, to the C-terminus of the gene of GFP, allows the secretion directly from the cytosol into the extracellular environment by the type 1 secretion system HlyB–HlyD–TolC complex. | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K3320013 SequenceAndFeatures</partinfo> | <partinfo>BBa_K3320013 SequenceAndFeatures</partinfo> | ||
Latest revision as of 00:52, 21 October 2019
GFPmut3 + HlyA
This is GFPmut3 based on BBa_E0040 with a secretion tag from the alpha-hemolysin system.
Usage and Biology
GFP is a fluorescent protein from Aequorea victoria. By fusing the HlyA sequence, a peptide consisting of 60 amino acids, to the C-terminus of the gene of GFP, allows the secretion directly from the cytosol into the extracellular environment by the type 1 secretion system HlyB–HlyD–TolC complex.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]