Difference between revisions of "Part:BBa K2978202"

Latest revision as of 18:12, 13 December 2019

B0015 Terminators Measurement in Lactobacillus spp

This part is the negative control for the efficiency terminators assay, when we tested three different sequences for Lactobacillus spp K2978201 K2978202 K2978203 K2978204 and K2978205.

The composite part is based in constitutively express a GFP before of each terminator tested, and a RFP in a polycistronic way after the terminator. By comparing the fluorescence of each protein, the efficiency of the terminator could be determined.

This specific terminator is descripted in the next link: https://parts.igem.org/Part:BBa_B0015

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2978202 short</partinfo>
-This part is the negative control for the efficiency terminators assay, where we tested three different sequences for <i>Lactobacillus spp</i> [[Part:BBa_K2978201| K2978201]] [[Part:BBa_K2978202| K2978202]] [[Part:BBa_K2978203| K2978203]] [[Part:BBa_K2978204| K2978204]] and [[Part:BBa_K2978205| K2978205]].
+This part is the negative control for the efficiency terminators assay, when we tested three different sequences for <i>Lactobacillus spp</i> [[Part:BBa_K2978201| K2978201]] [[Part:BBa_K2978202| K2978202]] [[Part:BBa_K2978203| K2978203]] [[Part:BBa_K2978204| K2978204]] and [[Part:BBa_K2978205| K2978205]].
 The composite part is based in constitutively express a GFP before of each terminator tested, and a RFP in a polycistronic way after the terminator. By comparing the fluorescence of each protein, the efficiency of the terminator could be determined.
+This specific terminator is descripted in the next link:
+https://parts.igem.org/Part:BBa_B0015
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 ===Usage and Biology===
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