Difference between revisions of "Part:BBa K3122006"

 
 
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Ptac, trp and lac regulated promoter (Part: <partinfo>BBa_K864400</partinfo>), adapted to MoClo standard by our team. It was designed to control Streptavidin-CBDs expression via IPTG induction.
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===Usage and Biology===
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<h2> Description </h2>
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Promotor Ptac is a combination of the different promoters from the lac and tpr operons. The advantages of this promoter include its high efficiency (11 times more than the lac one and 3 times more than the tpr one) and the fine control of the expression it allows (it can be repressed by lac repressor and derepressed with isopropyl-beta-D-thiogalactoside).
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<h2> Characterization </h2>
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Characterization of this part was planned to be conducted by building three transcriptional units ( <partinfo>BBa_K3122016</partinfo>, <partinfo>BBa_K3122017</partinfo>, <partinfo>BBa_K3122018</partinfo>) expressing different recombinant proteins. Unfortunately, no results were obtained.
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<h2> Sequence and Features </h2>
  
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<span class='h3bb'>Sequence and Features</span>
 
 
<partinfo>BBa_K3122006 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3122006 SequenceAndFeatures</partinfo>
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<h2> References </h2>
  
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de Boer, H., Comstock, L. and Vasser, M. (1983). The tac promoter: a functional hybrid derived from the trp and lac promoters. Proceedings of the National Academy of Sciences, 80(1), pp.21-25.
  
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===Functional Parameters===
 
 
<partinfo>BBa_K3122006 parameters</partinfo>
 
<partinfo>BBa_K3122006 parameters</partinfo>
 
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Latest revision as of 09:44, 22 November 2019


Inducible Ptac promoter (TypeIIS adapted)


Ptac, trp and lac regulated promoter (Part: BBa_K864400), adapted to MoClo standard by our team. It was designed to control Streptavidin-CBDs expression via IPTG induction.

Description

Promotor Ptac is a combination of the different promoters from the lac and tpr operons. The advantages of this promoter include its high efficiency (11 times more than the lac one and 3 times more than the tpr one) and the fine control of the expression it allows (it can be repressed by lac repressor and derepressed with isopropyl-beta-D-thiogalactoside).

Characterization

Characterization of this part was planned to be conducted by building three transcriptional units ( BBa_K3122016, BBa_K3122017, BBa_K3122018) expressing different recombinant proteins. Unfortunately, no results were obtained.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

de Boer, H., Comstock, L. and Vasser, M. (1983). The tac promoter: a functional hybrid derived from the trp and lac promoters. Proceedings of the National Academy of Sciences, 80(1), pp.21-25.