Difference between revisions of "Part:BBa K3245015"

 
(One intermediate revision by the same user not shown)
Line 10: Line 10:
  
 
<p>Please refer to https://parts.igem.org/Part:BBa_K3245001 </p>
 
<p>Please refer to https://parts.igem.org/Part:BBa_K3245001 </p>
 
 
  
 
__NOTOC__
 
__NOTOC__

Latest revision as of 11:41, 20 October 2019

Description:

This composite part is used for measurement of the new hybrid promoter.

Usage:

Transfer with high copy control plasmid containing luxI-luxR part ( BBa_K3245002 ) to test real time quorum sensing behavior and collect data such as OD600 and fluorescence. Transfer with plasmid containing luxR part ( BBa_C0062, etc. ) and start measuring with artificial AHL induction. It’s more suitable when accurate mechanism or dynamics research is needed.

Characterization:

Please refer to https://parts.igem.org/Part:BBa_K3245001


This composite part is used for measurement of the new promoter.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 26
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]




This composite part is used for measurement of the new hybrid promoter.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 26
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]