Difference between revisions of "Part:BBa K2970000"
 
(19 intermediate revisions by the same user not shown)
Line 3: Line 3:
 
<partinfo>BBa_K2970000 short</partinfo>
 
<partinfo>BBa_K2970000 short</partinfo>
  
−
Trigger 1 forms together with Trigger 2 (<partinfo>BBa_K2970001</partinfo>) a complex due to complementary regions. This complex can open a gate (<partinfo>BBa_K2970002</partinfo>) to enable translation of a gene that is locked with this gate to regulate translation. After binding of the trigger complex due to complementarity, the hairpin of the gate will open and release the ribosome binding site and the start codon, thus translation can occure.  
+
Trigger 1 forms together with Trigger 2 (<partinfo>BBa_K2970001</partinfo>) a complex due to complementary regions, as shown in Figure 1A. This complex can open a gate (<partinfo>BBa_K2970002</partinfo>, Figure 1B) to enable translation of a gene that is locked with this gate to regulate translation. After binding of the trigger complex due to complementarity, the hairpin of the gate will open and release the ribosome binding site and the start codon, thus translation can be initiated (Figure 2). The affinity between the trigger complex and the gate is greater than that of the gate to itself (in the hairpin). A single trigger cannot open the gate because it contains only half the required complementary sequence.
−
To transform the trigger into bacteria it should be put on a plasmid together with a promoter and a terminator as we did (<partinfo>BBa_K2970003</partinfo>).  
+
  
−
After transformation of both trigger plasmids and the gate plasmid in one bacterium all three mRNA structures will be formed, the gate will be opened, and the translation of the gene of interest can take place.
+
<html>
 +
<figure>
 +
<img width="100%" src="https://2019.igem.org/wiki/images/b/bc/T--Hamburg--PartsFigure11.jpeg">
 +
<figcaption>
 +
<b>Figure 1: </b>A) Formation of trigger complex after translation. B) mRNA of gate sequence forms secondary structures that hide the ribosome binding site and the start codon.
 +
</figcaption>
 +
</figure>
 +
</html>
 +
<html>
 +
<figure>
 +
<img width="90%" src="https://2019.igem.org/wiki/images/4/49/T--Hamburg--Part_Figure6.jpg">
 +
<figcaption>
 +
<b>Figure 2: </b>Opening of the gate due to annealing of trigger complex to gate.
 +
</figcaption>
 +
</figure>
 +
</html>
 +
To transform the trigger into bacteria it should be put on a plasmid together with a promoter and a terminator as we did (<partinfo>BBa_K2970003</partinfo>).
 +
After transformation of both trigger plasmids and the gate plasmid in one bacterium all three mRNA structures will be formed, the gate opens, and the translation of the gene of interest can be initiated.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 01:28, 22 October 2019


Trigger 1

Trigger 1 forms together with Trigger 2 (BBa_K2970001) a complex due to complementary regions, as shown in Figure 1A. This complex can open a gate (BBa_K2970002, Figure 1B) to enable translation of a gene that is locked with this gate to regulate translation. After binding of the trigger complex due to complementarity, the hairpin of the gate will open and release the ribosome binding site and the start codon, thus translation can be initiated (Figure 2). The affinity between the trigger complex and the gate is greater than that of the gate to itself (in the hairpin). A single trigger cannot open the gate because it contains only half the required complementary sequence.

Figure 1: A) Formation of trigger complex after translation. B) mRNA of gate sequence forms secondary structures that hide the ribosome binding site and the start codon.
Figure 2: Opening of the gate due to annealing of trigger complex to gate.
To transform the trigger into bacteria it should be put on a plasmid together with a promoter and a terminator as we did (BBa_K2970003). After transformation of both trigger plasmids and the gate plasmid in one bacterium all three mRNA structures will be formed, the gate opens, and the translation of the gene of interest can be initiated.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]